Ramanathan, Babu * and Poh, Chit Laa * and Kirk, Kristin and McBride, William John Hannan and Aaskov, John and Grollo, Lara (2016) Synthetic B-Cell epitopes eliciting cross-neutralizing antibodies: Strategies for future dengue vaccine. PLOS ONE, 11 (5). e0155900. ISSN 1932-6203
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Abstract
Dengue virus (DENV) is a major public health threat worldwide. A key element in protection from dengue fever is the neutralising antibody response. Anti-dengue IgG purified from DENV-2 infected human sera showed reactivity against several peptides when evaluated by ELISA and epitope extraction techniques. A multi-step computational approach predicted six antigenic regions within the E protein of DENV-2 that concur with the 6 epitopes identified by the combined ELISA and epitope extraction approach. The selected peptides representing B-cell epitopes were attached to a known dengue T-helper epitope and evaluated for their vaccine potency. Immunization of mice revealed two novel synthetic vaccine constructs that elicited good humoral immune responses and produced cross-reactive neutralising antibodies against DENV-1, 2 and 3. The findings indicate new directions for epitope mapping and contribute towards the future development of multi-epitope based synthetic peptide vaccine.
| Item Type: | Article |
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| Additional Information: | 3rd author is with Swinburne University of Technology, Melbourne; 4th author is with James Cook University Clinical School; 5th author is with Queensland University of Technology; 6th author is Australian Catholic University |
| Subjects: | Q Science > QR Microbiology R Medicine > R Medicine (General) |
| Divisions: | Others > Non Sunway Academics Sunway University > School of Medical and Life Sciences [formerly School of Healthcare and Medical Sciences until 2020] > Centre of Biomedical Physics [wef 2021 changed to Research Centre for Applied Physics and Radiation Technologies and moved to SET ] |
| Depositing User: | Ms. Molly Chuah |
| Related URLs: | |
| Date Deposited: | 20 Jan 2017 08:12 |
| Last Modified: | 04 Jul 2019 08:04 |
| URI: | http://eprints.sunway.edu.my/id/eprint/454 |
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