Sumayah, Abdelnasir * and Mohammad Ridwane, Mungroo * and Shahabuddin, Syed and Siddiqui, Ruqaiyyah and Khan, Naveed Ahmed and Ahmad, Irfan and Ayaz, Anwar * (2022) Polyaniline (PANI)-conjugated tungsten disulphide (WS2) nanoparticles as potential therapeutics against brain-eating amoebae. Applied Microbiology and Biotechnology, 106. pp. 3279-3291. ISSN 0175 7598
Full text not available from this repository.Abstract
Brain-eating amoebae, including Acanthamoeba castellanii and Naegleria fowleri, are the causative agents of devastating central nervous system infections with extreme mortality rates. There is an indisputable urgency for the development of effective chemotherapeutic agents for the control of these diseases that are increasing in incidence. Here, we evaluated the anti-amoebic potential of polyaniline:tungsten disulphide (PANI:WS2) nanocomposite against the infective trophozoite and cyst stages of N. fowleri and A. castellanii. Throughout these evaluations, significant viability inhibition was noted when 100 µg/mL of PANI:WS2 was employed at its 1:5 formulation. These effects were studied to be due to increased levels of reactive oxygen species (ROS) as visualised through fluorescence microscopy. Furthermore, field emission scanning electron microscopy (FE-SEM) analysis pictured disruption to amoeba morphology. The host-cell cytotoxicity of the nanocomposite (PANI:WS2) was studied to be negligible, making it an attractive avenue in the pursuit for effective treatments for brain-eating amoeba infections. KEY POINTS: • Synthesis of polyaniline:tungsten disulphide (PANI:WS2) nanocomposite. • Anti-amoebic potential of PANI:WS2 nanocomposite. • PANI:WS2 nanocomposites are promising anti-amoebic agents in vitro.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | anti-amoebic; brain-eating amoeba; nanoparticles; PANI; polyaniline; tungsten disulphide; |
| Subjects: | R Medicine > RA Public aspects of medicine R Medicine > RC Internal medicine R Medicine > RM Therapeutics. Pharmacology |
| Divisions: | Others > Non Sunway Academics Sunway University > School of Medical and Life Sciences [formerly School of Healthcare and Medical Sciences until 2020] > Dept. Biological Sciences |
| Depositing User: | Ms Yong Yee Chan |
| Date Deposited: | 14 Aug 2024 00:42 |
| Last Modified: | 14 Aug 2024 00:42 |
| URI: | http://eprints.sunway.edu.my/id/eprint/3134 |
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