Goh, Calvin Bok Sun and Goh, Clariss Hui Peng and Wong, Li Wen and Cheng, Wai Teng and Yule, Catherine Mary and Ong, Kuan Shion and Lee, Sui Mae and Pooria, Pasbakhsh * and Tan, Joash Ban Lee (2022) A three-dimensional (3D) printing approach to fabricate an isolation chip for high throughput in situ cultivation of environmental microbes. Lab on a Chip, 22 (2). ISSN 1473-0197
Full text not available from this repository.Abstract
The full plethora of environmental bacteria is often poorly represented in vitro as the majority remain difficult, if not impossible, to culture under standard laboratory settings. These bacteria often require native conditions for the formation of cell masses that collectively have higher chances of survival. With that, a 3D-printed version of the isolation chip (iChip) was used to cultivate bacteria from a tropical peat swamp in situ prior to growth and maintenance in vitro. Briefly, plates made from either acrylonitrile butadiene styrene (ABS), polylactic acid (PLA), or epoxy resin were tested in terms of their usability and durability under acidic conditions similar to those of peat matter. The epoxy resin plates were then found to be most optimal for the sampling conditions. Peat soil samples were collected from the base of a Koompassia malaccensis tree and reconstituted in molten 10% (wt/vol) tryptone soy agar (TSA) prior to inoculation. The iChips were subsequently assembled and buried in the site of origin. As a comparison, bacteria from the same soil sample were cultivated directly on TSA and incubated at 28 °C for two weeks. Thereafter, agar plugs from the iChip were transferred to TSA plates to allow microcolonies within each plug to grow. Each pure isolate from both cultivation approaches that grew was then pooled and extracted for total DNA prior to 16S rRNA gene amplification and sequencing via Illumina MiSeq. Taxonomic abundance comparison revealed that the bacterial taxa at the level of order were significantly different between the two approaches, particularly in the orders, Burkholderiales, Xanthomonodales, Enterobacteriales, and Actinomycetales (differences of 12.0, 7.1, 8.0, and 4.2%, respectively). This indicated that the 3D-printed iChips present a possible low-cost tool for the isolation of bacterial genera that may not be able to grow on media directly in vitro.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | plethora; cultivate bacteria; 3D-printed iChips; acrylonitrile butadiene styrene; polylactic acid; iChips; |
| Subjects: | Q Science > QR Microbiology R Medicine > RC Internal medicine T Technology > TS Manufactures |
| Divisions: | Others > Non Sunway Academics Sunway University > School of Medical and Life Sciences [formerly School of Healthcare and Medical Sciences until 2020] > Department of Medical Sciences |
| Depositing User: | Ms Yong Yee Chan |
| Date Deposited: | 06 Aug 2024 06:00 |
| Last Modified: | 06 Aug 2024 06:00 |
| URI: | http://eprints.sunway.edu.my/id/eprint/3014 |
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