Liu, Lanbo and Gopinath, Subash C B and Wu, Yuan Seng * and Zhao, Weichao (2022) Gold-enhanced current-volt dielectrode junction for biosensing with an aptamer-insulin-like growth factor-1-antibody sandwich pattern. Materials Express, 12 (3). ISSN 2158-5849
Full text not available from this repository. (Request a copy)Abstract
Insulin-like growth factor-1 (IGF-1) is a hormone comprising seventy amino acids that is a key regulator of muscle and bone health and is highly related to the development of cancer. An aptamer-antibody sandwich assay was performed to determine the presence of IGF1 on a gold nanoparticle-enhanced dielectrode junctional sensing surface by using this surface as a transducer. High-resolution microscopy observations showed the characteristics of the sensing surface and gold nanoparticles. The gold nanoparticle-enhanced surface induces high immobilization of the anti-IGF1 aptamer and enhances the interactions of IGF1. With this probe surface, the aptamer-IGF1-antibody sandwich assay resulted in an increased current for each different solution concentration of IGF1, and the detection limit was 0.5 pg/mL with an R 2 value of 0.9631 on a linear calibration curve with IGF1 solution concentrations of 1–16 pg/mL. In addition, IGF1-spiked serum reached a similar limit of detection of 0.5 pg/mL, confirming that selective IGF1 detection occurred for the biological sample.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | abdominal aortic aneurysm; biomarker; dipole moment; nanomaterial; nanosensor |
| Subjects: | Q Science > QC Physics Q Science > QH Natural history R Medicine > R Medicine (General) |
| Divisions: | Others > Non Sunway Academics Sunway University > School of Medical and Life Sciences [formerly School of Healthcare and Medical Sciences until 2020] > Sunway Microbiome Centre [formerly Centre for Virus and Vaccine Research until 2023] |
| Depositing User: | Ms Yong Yee Chan |
| Related URLs: | |
| Date Deposited: | 04 Aug 2024 02:01 |
| Last Modified: | 04 Aug 2024 02:01 |
| URI: | http://eprints.sunway.edu.my/id/eprint/2964 |
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