Elouafy, Youssef and El Yadini, Adil and Mortada, Salma and Hnini, Mohamed and Harhar, Hicham and Khalid, Asaad and Abdalla, Ashraf N. and Bouyahya, Abdelhakim and Goh, Khang Wen and Long, Chiau Ming * and Faouzi, My El Abbes and Tabyaoui, Mohamed (2023) Antioxidant, antimicrobial, and α-glucosidase inhibitory activities of saponin extracts from walnut (Juglans regia L.) leaves. Asian Pacific Journal of Tropical Biomedicine, 13 (2). pp. 60-69. ISSN 2221-1691
Full text not available from this repository. (Request a copy)Abstract
Objective: To investigate the relationship between triterpenoid saponin content and antioxidant, antimicrobial, and α-glucosidase inhibitory activities of 70% ethanolic, butanolic, aqueous, supernate and precipitate extracts of Juglans regia leaves. Methods: Triterpenoid saponins of different Juglans regia leaf extracts were measured by the vanillin method. Antioxidant activity was evaluated against DPPH and ABTS free radicals. We also assessed α-glucosidase inhibitory and antimicrobial activities of the leaf extracts. Pearson's correlation coefficient was evaluated to determine the correlation between the saponin content and biological activities. Results: The butanolic extract was most effective against DPPH with an IC50 of 6.63 μg/mL, while the aqueous extract showed the highest scavenging activity against ABTS free radical with an IC50 of 42.27 μg/mL. Pearson's correlation analysis indicated a strong negative correlation (r = -0.956) between DPPH radical scavenging activity (IC50) and the saponin content in the samples examined. In addition, the aqueous extract showed the best α-glucosidase inhibitory activity compared with other extracts. All the extracts had fair antibacterial activity against Bacillus subtilis, Escherichia coli, and Klebsiella pneumoniae except for the aqueous extract. Conclusions: Juglans regia extracts show potent antioxidant, antimicrobial, and α-glucosidase inhibitory activities. There is a correlation between saponin levels in Juglans regia leaf extracts and the studied activities. However, additional research is required to establish these relationships by identifying the specific saponin molecules responsible for these activities and elucidating their mechanisms of action.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | triterpenoid saponin; antioxidant; antimicrobial; Juglans regia; |
| Subjects: | Q Science > QK Botany R Medicine > RS Pharmacy and materia medica T Technology > TP Chemical technology |
| Divisions: | Others > Non Sunway Academics Sunway University > School of Medical and Life Sciences [formerly School of Healthcare and Medical Sciences until 2020] > Department of Medical Sciences |
| Depositing User: | Ms Yong Yee Chan |
| Related URLs: | |
| Date Deposited: | 26 Jul 2024 01:01 |
| Last Modified: | 26 Jul 2024 01:01 |
| URI: | http://eprints.sunway.edu.my/id/eprint/2896 |
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