El-Guourrami, Otman and Salhi, Najoua and Fatima, Zahra and Zengin, Gokhan and Yilmaz, Mustafa Abdullah and Ameggouz, Mouna and Zahidi, Ahmed and Rouas, Lamiaa and Bouyahya, Abdelhakim and Goh, Khang Wen and Sam, Toong Hai and Long, Chiau Ming * and Doukkali, Anass and Benzeid, Hanane (2023) Phytochemical composition and toxicity assessment of Ammi majus L. Asian Pacific Journal of Tropical Biomedicine, 13 (4). pp. 165-175. ISSN 2221-1691
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Objective: To assess the acute and subacute toxicity as well as the phytochemical composition of two extracts and three fractions of Ammi majus L. Methods: The aqueous extracts were prepared separately by maceration for 48 h and by infusion for 1 h, while the fractions were prepared by the Soxhlet extractor, successively employing cyclohexane, ethyl acetate, and ethanol. The acute toxicity study was carried out in accordance with the OECD N°423 guideline at a single dose (2000 mg/kg) in mice for 14 days. The subacute toxicity study was performed by a daily oral administration of 250 mg/kg for 10 days and 100 mg/kg doses for 28 days. Phytochemical screening was performed using staining and precipitation reactions, while the chemical characterization of some analytes was detected by HPLC-MS/MS analysis. Results: In the acute toxicity study, no signs of toxicity such as convulsion, salivation, diarrhea, sleep and coma were observed during 30 minutes and 14 days, so the lethal dose was higher than 2000 mg/kg for each extract and fraction. The subacute toxicity results showed that at a dose of 250 mg/kg, 61.10% of the animals died and the rest developed morbidity. On the other hand, at a dose of 100 mg/kg, all the animals were still alive after 28 days, with no morbidity and the biochemical parameters were normal with no abnormalities in the liver, kidneys and pancreas. Phytochemical screening indicated the presence of flavonoids, tannins, coumarins, and free quinones and the absence of alkaloids and anthocyanins. Conclusions: The extracts and fractions of Ammi majus L. are not toxic in the short and long term with a varied chemical composition. Toxicological tests on animals other than rodents and in the long term (more than 28 days) are needed to further confirm the safety of Ammi majus extracts.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | phytochemical; toxicity; Ammi majus; traditional medicine; |
| Subjects: | Q Science > QH Natural history Q Science > QK Botany R Medicine > RA Public aspects of medicine |
| Divisions: | Others > Non Sunway Academics Sunway University > School of Medical and Life Sciences [formerly School of Healthcare and Medical Sciences until 2020] > Department of Medical Sciences |
| Depositing User: | Ms Yong Yee Chan |
| Related URLs: | |
| Date Deposited: | 26 Jul 2024 00:10 |
| Last Modified: | 26 Jul 2024 00:10 |
| URI: | http://eprints.sunway.edu.my/id/eprint/2892 |
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