Kanwal, Khalid * and Poh, Chit Laa * (2023) The development of DNA vaccines against SARS-CoV-2. Advances in Medical Sciences, 68 (2). pp. 213-226. ISSN 1898-4002
Full text not available from this repository.Abstract
Background The COVID-19 pandemic exerted significant impacts on public health and global economy. Research efforts to develop vaccines at warp speed against SARS-CoV-2 led to novel mRNA, viral vectored, and inactivated vaccines being administered. The current COVID-19 vaccines incorporate the full S protein of the SARS-CoV-2 Wuhan strain but rapidly emerging variants of concern (VOCs) have led to significant reductions in protective efficacies. There is an urgent need to develop next-generation vaccines which could effectively prevent COVID-19. Methods PubMed and Google Scholar were systematically reviewed for peer-reviewed papers up to January 2023. Results A promising solution to the problem of emerging variants is a DNA vaccine platform since it can be easily modified. Besides expressing whole protein antigens, DNA vaccines can also be constructed to include specific nucleotide genes encoding highly conserved and immunogenic epitopes from the S protein as well as from other structural/non-structural proteins to develop effective vaccines against VOCs. DNA vaccines are associated with low transfection efficiencies which could be enhanced by chemical, genetic, and molecular adjuvants as well as delivery systems. Conclusions The DNA vaccine platform offers a promising solution to the design of effective vaccines. The challenge of limited immunogenicity in humans might be solved through the use of genetic modifications such as the addition of nuclear localization signal (NLS) peptide gene, strong promoters, MARs, introns, TLR agonists, CD40L, and the development of appropriate delivery systems utilizing nanoparticles to increase uptake by APCs in enhancing the induction of potent immune responses.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | DNA vaccine; SARS-CoV-2; variants; vaccine efficacy; plasmid design; |
| Subjects: | Q Science > QR Microbiology R Medicine > RA Public aspects of medicine R Medicine > RM Therapeutics. Pharmacology |
| Divisions: | Sunway University > School of Medical and Life Sciences [formerly School of Healthcare and Medical Sciences until 2020] > Centre for Virus and Vaccine Research [dissolved] |
| Depositing User: | Ms Yong Yee Chan |
| Date Deposited: | 16 Jul 2024 01:53 |
| Last Modified: | 16 Jul 2024 01:53 |
| URI: | http://eprints.sunway.edu.my/id/eprint/2825 |
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