Epstein-Barr Virus- (EBV-) immortalized Lymphoblastoid Cell Lines (LCLs) express high level of CD23 but low CD27 to support their growth

Yap, Hooi Yeen * and Siow, Thin Sam and Chow, Sook Khuan * and Teow, Sin Yeang * (2019) Epstein-Barr Virus- (EBV-) immortalized Lymphoblastoid Cell Lines (LCLs) express high level of CD23 but low CD27 to support their growth. Advances in Virology, 2019. pp. 1-9. ISSN 1687-8639

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Abstract

Epstein-Barr virus (EBV) is one of the common human herpesvirus types in the world. EBV is known to infect more than 95% of adults in the world. Te virus mainly infects B lymphocytes and could immortalize and transform the cells into EBVbearing lymphoblastoid cell lines (LCLs). Limited studies have been focused on characterizing the surface marker expression of the immortalized LCLs. Tis study demonstrates the generation of 15 LCLs from sixteen rheumatoid arthritis (RA) patients and a healthy volunteer using B95-8 marmoset-derived EBV. Te success rate of LCL generation was 88.23%. All CD19+ LCLs expressed CD23 (16.94-58.9%) and CD27 (15.74-80.89%) on cell surface. Our data demonstrated two distinct categories of LCLs (fast- and slow-growing) (p<0.05) based on their doubling time. Te slow-growing LCLs showed lower CD23 level (35.28%) compared to fast-growing LCLs (42.39%). In contrast, the slow-growing LCLs showed higher percentage in both CD27 alone and CD23+CD27+ in combination. Overall, these fndings may suggest the correlations of cellular CD23 and CD27 expression with the proliferation rate of the generated LCLs. Increase expression of CD23 may play a role in EBV immortalization of B-cells and the growth and maintenance of the EBV-transformed LCLs while CD27 expression might have inhibitory efects on LCL proliferation. Further investigations are warranted to these speculations.

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