Synthetic B-Cell epitopes eliciting cross-neutralizing antibodies: Strategies for future dengue vaccine

Ramanathan, Babu * and Poh, Chit Laa * and Kirk, Kristin and McBride, William John Hannan and Aaskov, John and Grollo, Lara (2016) Synthetic B-Cell epitopes eliciting cross-neutralizing antibodies: Strategies for future dengue vaccine. PLOS ONE, 11 (5). e0155900. ISSN 1932-6203

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Official URL: http://dx.doi.org/10.1371/journal.pone.0155900

Abstract

Dengue virus (DENV) is a major public health threat worldwide. A key element in protection from dengue fever is the neutralising antibody response. Anti-dengue IgG purified from DENV-2 infected human sera showed reactivity against several peptides when evaluated by ELISA and epitope extraction techniques. A multi-step computational approach predicted six antigenic regions within the E protein of DENV-2 that concur with the 6 epitopes identified by the combined ELISA and epitope extraction approach. The selected peptides representing B-cell epitopes were attached to a known dengue T-helper epitope and evaluated for their vaccine potency. Immunization of mice revealed two novel synthetic vaccine constructs that elicited good humoral immune responses and produced cross-reactive neutralising antibodies against DENV-1, 2 and 3. The findings indicate new directions for epitope mapping and contribute towards the future development of multi-epitope based synthetic peptide vaccine.

Item Type: Article
Additional Information: 3rd author is with Swinburne University of Technology, Melbourne; 4th author is with James Cook University Clinical School; 5th author is with Queensland University of Technology; 6th author is Australian Catholic University
Subjects: Q Science > QR Microbiology
R Medicine > R Medicine (General)
Divisions: Others > Non Sunway Academics
Sunway University > Research Centres > Research Centre for Biomedical Sciences
Depositing User: Ms. Molly Chuah
Date Deposited: 20 Jan 2017 08:12
Last Modified: 20 Jan 2017 08:12
URI: http://eprints.sunway.edu.my/id/eprint/454

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