Novel genetic variants of Hepatitis B Virus in fulminant hepatitis

Chook, Jack Bee * and Ngeow, Yun Fong and Tee, Kok Keng and Peh, Suat Cheng * and Mohamed, Rosmawati (2017) Novel genetic variants of Hepatitis B Virus in fulminant hepatitis. Journal of Pathogens, 2017. pp. 1-6. ISSN 2090-3057

Preview

Text Chook JB 2017 Novel Genetic variants of fulminant HBV J Pathog.pdf Download (1MB) | Preview

Abstract

Fulminant hepatitis (FH) is a life-threatening liver disease characterised by intense immune attack and massive liver cell death. The common precore stop codon mutation of hepatitis B virus (HBV), A1896, is frequently associated with FH, but lacks specificity. This study attempts to uncover all possible viral nucleotides that are specifically associated with FH through a compiled sequence analysis of FH and non-FH cases from acute infection. We retrieved 67 FH and 280 acute non-FH cases of hepatitis B from GenBank and applied support vector machine (SVM) model to seek candidate nucleotides highly predictive of FH. Six best candidates with top predictive accuracy, 92.5%, were used to build a SVM model; they are C2129 (85.3%), T720 (83.0%), Y2131 (82.4%), T2013 (82.1%),K2048 (82.1%), and A2512 (82.1%). This model gave a high specificity (99.3%), positive predictive value (95.6%), and negative predictive value (92.1%), but only moderate sensitivity (64.2%).We successfully built a SVM model comprising six variants that are highly predictive and specific for FH: four in the core region and one each in the polymerase and the surface regions. These variants indicate that intracellular virion/core retention could play an important role in the progression to FH.

Actions (login required)

View Item