Energetic mixing of anti-SNAP25 on lipid monolayers: degree of saturation of C18 fatty acids

Gew, Lai Ti * and Misni Misran, (2016) Energetic mixing of anti-SNAP25 on lipid monolayers: degree of saturation of C18 fatty acids. Surface and Interface Analysis, 49 (5). pp. 388-397. ISSN 01422421

Energetic mixing of anti-SNAP25 on lipid monolayers (Sunway).pdf - Accepted Version
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Official URL: http://dx.doi.org/10.1002/sia.6144


In our study, various mixtures of C18 fatty acids with different degrees of saturation in their hydrocarbon chain, namely stearic acid (SA), oleic acid (L1), linoleic acid (L2), and linolenic acid (L3), and a polyclonal antibody, anti-synaptosome-associated protein of 25 kDa (SNAP25) (AS25), have been investigated using the Langmuir–Blodgett (LB) technique accompanied by atomic force microscopy (AFM) imaging. The cis-double bonds in unsaturated lipids (L1, L2, and L3) have kinks in their molecular conformation and thus could not pack as tightly and uniformly as SA. The bends and kinks in the molecular structure may interfere with the packing of the lipid monolayer which will promote fluidity as shown in the analyzed compressibility modulus (Cs−1) data. The negative values of Gibbs free energy of mixing (ΔGmix) of C18 fatty acids/AS25 confirm the spontaneity interaction of AS25 molecules on the monolayers. The amount of AS25 incorporated into the monolayer strongly affected the thermodynamic properties of the lipid monolayers. AFM surface roughness analyses also indicate that AS25 molecules are strongly bounded on the surface membrane as predicted by the obtained energetic data. In comparison to all C18 fatty acids studied, the strongest intermolecular interaction is observed in L1 at the investigated ranges. In particular, at mole ratio of 26:1, the most negative ΔGmix is observed at L1. Thus, we can draw the conclusion that AS25 is best mixed with L1. This L1/AS25 ratio mimicking a half bilayer membrane serves as a very useful reference in preparing fatty-acid nanoimmunoliposomes as the targeted drug-delivery vehicles for cancer therapy

Item Type: Article
Additional Information: First author is with the Dept. Biological Sciences, Faculty of Science and Technology, Sunway University; 2nd author is with the Department of Chemistry, Faculty of Science, University of Malaya
Uncontrolled Keywords: lipid–protein interactions; Langmuir–Blodgett technique; atomic force microscopy; intermolecular interactions; lipid bilayer; surface roughness
Subjects: Q Science > QD Chemistry
Divisions: Others > Non Sunway Academics
Sunway University > School of Engineering and Technology [formerly School of Science and Technology until 2020] > Dept. Biological Sciences moved to SMLS wef 2021
Depositing User: Ms. Molly Chuah
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Date Deposited: 30 Oct 2017 05:23
Last Modified: 25 Apr 2019 07:01
URI: http://eprints.sunway.edu.my/id/eprint/635

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