Yousuf, Farzana Abubakar and Rafiq, Sahar and Siddiqui, Ruqaiyyah Bano * and Khan, Naveed Ahmed * (2016) The role of genomic islands in Escherichia coli K1 interactions with intestinal and kidney epithelial cells. Microbial Pathogenesis, 93. pp. 145-151. ISSN 08824010
Full text not available from this repository.Abstract
The completion of Escherichia coli K1 genome has identified several genomic islands that are present in meningitis-causing E. coli RS218 but absent in the non-pathogenic E. coli MG1655. In this study, the role of various genomic islands in E. coli K1 interactions with intestinal epithelial cells (Caco-2) and kidney epithelial cells (MA104) was determined. Using association assays, invasion assays, and intracellular survival assays, the findings revealed that the genomic island deletion mutants of RS218 related to P fimbriae, S fimbriae, F17-like fimbriae, non-fimbrial adhesins, Hek and hemagglutinin, protein secretion system (T1SS for hemolysin; T2SS; T5SS for antigen 43), Iro system and hmu system), invasins (CNF1, IbeA), toxins (a-hemolysin), K1 capsule biosynthesis, metabolism (D-serine catabolism, dihydroxyacetone, glycerol, and glyoxylate metabolism), prophage genes, showed reduced interactions with both cell types. Next, we determined the role of various genomic islands in E. coli K1 resistance to serum. When exposed to the normal human serum, the viability of the genomic island deletion mutants related to adhesins such as S fimbriae, P fimbriae, F17-like fimbriae, non-fimbrial adhesins, Hek and hemagglutinin, antigen 43 and T5SS for antigen 43, T2SS, and T1SS for hemolysin, Iro system and hmu system, prophage genes, metabolism (sugar metabolism and D-serine catabolism), K1 capsule biosynthesis, and invasins such as CNF1 was affected, suggesting their role in bacteremia. The characterization of these genomic islands should reveal mechanisms of E. coli K1 pathogenicity that could be of value as therapeutic targets.
| Item Type: | Article |
|---|---|
| Additional Information: | First and 2nd authors are with Dept. of Biological and Biomedical Sciences, Aga Khan University; 3rd and 4th authors are with Dept. of Biological Sciences, Faculty of Science and Technology, Sunway University |
| Uncontrolled Keywords: | bacteria; pathogenicity; invasion; survival association; epithelial cells |
| Subjects: | Q Science > QR Microbiology |
| Divisions: | Others > Non Sunway Academics Sunway University > School of Engineering and Technology [formerly School of Science and Technology until 2020] > Research Centre for Crystalline Materials moved to SMLS wef 2021 |
| Depositing User: | Ms. Molly Chuah |
| Related URLs: | |
| Date Deposited: | 26 Oct 2017 08:32 |
| Last Modified: | 23 Apr 2019 01:20 |
| URI: | http://eprints.sunway.edu.my/id/eprint/624 |
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