Targeting cyst wall is an effective strategy in improving the efficacy of marketed contact lens disinfecting solutions against Acanthamoeba castellanii cysts

Abjani, Farhat and Khan, Naveed Ahmed * and Yousuf, Farzana Abubakar and Siddiqui, Ruqaiyyah Bano * (2016) Targeting cyst wall is an effective strategy in improving the efficacy of marketed contact lens disinfecting solutions against Acanthamoeba castellanii cysts. Contact Lens and Anterior Eye, 39 (3). pp. 239-243. ISSN 13670484

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Official URL: http://dx.doi.org/10.1016/j.clae.2015.11.004

Abstract

Acanthamoeba cysts are highly resistant to contact lens disinfecting solutions. Acanthamoeba cyst wall is partially made of 1,4 β-glucan (i.e., cellulose) and other complex polysaccharides making it a hardy shell that protects the resident amoeba. Here, we hypothesize that targeting the cyst wall structure in addition to antiamoebic compound would improve the efficacy of marketed contact lens disinfecting solutions. Using chlorhexidine as an antiamoebic compound and cellulase enzyme to disrupt cyst wall structure, the findings revealed that combination of both agents abolished viability of Acanthamoeba castellanii cysts and trophozoites. When tested alone, none of the agents nor contact lens disinfecting solutions completely destroyed A. castellanii cysts and trophozoites. The absence of cyst wall-degrading enzymes in marketed contact lens disinfecting solutions render them ineffective against Acanthamoeba cysts. It is concluded that the addition of cyst wall degrading molecules in contact lens disinfecting solutions will enhance their efficacy in decreasing the incidence of Acanthamoeba effectively.

Item Type: Article
Additional Information: First and 3rd authors are with Dept. of Biological and Biomedical Sciences, Aga Khan University, Pakistan; 2nd and 4th authors are with Dept. of Biological Sciences, Faculty of Science and Technology, Sunway University.
Uncontrolled Keywords: keratitis; contact lens; disinfecting solution; cellulase
Subjects: Q Science > QR Microbiology
Divisions: Others > Non Sunway Academics
Sunway University > School of Science and Technology > Dept. Biological Sciences
Depositing User: Ms. Molly Chuah
Related URLs:
Date Deposited: 26 Oct 2017 08:04
Last Modified: 23 Apr 2019 01:20
URI: http://eprints.sunway.edu.my/id/eprint/623

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