Identification and characterization of antibacterial compound(s) of cockroaches (Periplaneta americana)

Ali, Salwa Mansur * and Siddiqui, Ruqaiyyah Bano * and Ong, Seng Kai * and Shah, Mohammad Raza and Anwar, Ayaz and Heard , Peter J * and Khan, Naveed Ahmed * (2017) Identification and characterization of antibacterial compound(s) of cockroaches (Periplaneta americana). Applied Microbiology and Biotechnology, 101. pp. 253-286. ISSN 0175 7598

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Abstract

Infectious diseases remain a significant threat to human health, contributing to more than 17 million deaths, annually. With the worsening trends of drug resistance, there is a need for newer and more powerful antimicrobial agents. We hypothesized that animals living in polluted environments are potential source of antimicrobials. Under polluted milieus, organisms such as cockroaches encounter different types of microbes, including superbugs. Such creatures survive the onslaught of superbugs and are able to ward off disease by producing antimicrobial substances. Here, we characterized antibacterial properties in extracts of various body organs of cockroaches (Periplaneta americana) and showed potent antibacterial activity in crude brain extract against methicillin-resistant Staphylococcus aureus and neuropathogenic E. coli K1. The size-exclusion spin columns revealed that the active compound(s) are less than 10 kDa in molecular mass. Using cytotoxicity assays, it was observed that pre-treatment of bacteria with lysates inhibited bacteria-mediated host cell cytotoxicity. Using spectra obtained with LC-MS on Agilent 1290 infinity liquid chromatograph, coupled with an Agilent 6460 triple quadruple mass spectrometer, tissues lysates were analyzed. Among hundreds of compounds, only a few homologous compounds were identified that contained isoquinoline group, chromene derivatives, thiazine groups, imidazoles, pyrrole containing analogs, sulfonamides, furanones, flavanones, and known to possess broad-spectrum antimicrobial properties, and possess anti-inflammatory, anti-tumour, and analgesic properties. Further identification, characterization and functional studies using individual compounds can act as a breakthrough in developing novel therapeutics against various pathogens including superbugs.

Item Type: Article
Additional Information: (i) Short Title of article: "Antibacterial compound(s) of cockroaches" (ii) Authors no 4 & 5 are from H.E.J. Research Institute of Chemistry University of Karachi, Pakistan.
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QL Zoology
Q Science > QR Microbiology
Divisions: Others > Non Sunway Academics
Sunway University > Research Office
Sunway University > School of Science and Technology > Dept. Biological Sciences
Depositing User: Dr Janaki Sinnasamy
Date Deposited: 31 Jan 2017 07:16
Last Modified: 08 Feb 2017 08:53
URI: http://eprints.sunway.edu.my/id/eprint/464

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