Pluripotent human embryonic stem cell derived neural lineages for in vitro modelling of enterovirus 71 infection and therapy

Yap, May Shin * and Tang, Yin Quan * and Yeo, Yin * and Lim, Wei Ling * and Lim, Lee Wei and Tan, Kuan Onn * and Richards, Mark and Othman, Lekhsan and Poh, Chit Laa * and Heng, Alexis Boon Chin * (2016) Pluripotent human embryonic stem cell derived neural lineages for in vitro modelling of enterovirus 71 infection and therapy. Virology Journal, 13 (5). pp. 1-10. ISSN 1743-422X

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The incidence of neurological complications and fatalities associated with Hand, Foot & Mouth disease has increased over recent years, due to emergence of newly-evolved strains of Enterovirus 71 (EV71). In the search for new antiviral therapeutics against EV71, accurate and sensitive in vitro cellular models for preliminary studies of EV71 pathogenesis is an essential prerequisite, before progressing to expensive and time-consuming live animal studies and clinical trials. This study thus investigated whether neural lineages derived from pluripotent human embryonic stem cells (hESC) can fulfil this purpose. EV71 infection of hESC-derived neural stem cells (NSC) and mature neurons (MN) was carried out in vitro, in comparison with RD and SH-SY5Y cell lines. Results: Upon assessment of post-infection survivability and EV71 production by the various types, it was observed that NSC were significantly more susceptible to EV71 infection compared to MN, RD (rhabdomyosarcoma) and SHSY5Y cells, which was consistent with previous studies on mice. The SP81 peptide had significantly greater inhibitory effect on EV71 production by NSC and MN compared to the cancer-derived RD and SH-SY5Y cell lines. Hence, this study demonstrates that hESC-derived neural lineages can be utilized as in vitro models for studying EV71 pathogenesis and for screening of antiviral therapeutics.

Item Type: Article
Uncontrolled Keywords: EV71; HFMD; infectious diseases; neurons; viral
Subjects: Q Science > QR Microbiology > QR355 Virology
Divisions: Others > Non Sunway Academics
Sunway University > School of Engineering and Technology [formerly School of Science and Technology until 2020] > Dept. Biological Sciences moved to SMLS wef 2021
Sunway University > School of Medical and Life Sciences [formerly School of Healthcare and Medical Sciences until 2020] > Centre of Biomedical Physics [wef 2021 changed to Research Centre for Applied Physics and Radiation Technologies and moved to SET ]
Depositing User: Dr Janaki Sinnasamy
Related URLs:
Date Deposited: 29 Sep 2016 09:04
Last Modified: 17 Oct 2019 09:51

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