Nurneqman, Nashreq Kosni and Noraini, Abd-Aziz * and Abdul Rhaman, Omar and Saila, Ismail and Chia, Suet Lin (2023) Oncolytic effect of Newcastle disease virus (NDV) strain AF2240 and V4-UPM on hypoxic and normoxic osteosarcoma cells. Malaysian Journal of Microbiology, 19 (4). pp. 371-379. ISSN 2231-7538
Full text not available from this repository.Abstract
Aims: Hypoxia is believed to be one of the key components contributing to the clinical resistance of cancer therapies. Alternative strategies are under investigation to overcome this resistance and the oncolytic virus stands amongst the others. Newcastle disease virus (NDV) has been demonstrated to possess oncolytic activity against cancer cells. The present study investigated the effects of oncolytic NDV strain AF2240 and V4-UPM on osteosarcoma cells (Saos-2) under normoxic and hypoxic conditions. Methodology and results: Results showed that the NDV strain AF2240 and V4-UPM could infect and kill normoxic and hypoxic Saos-2 cells equally well by inducing hypoxia-independent apoptosis, and S-phase cell cycle arrest under the microscopy examination, cell viability assay, Annexin V apoptosis assay and cell cycle analysis experiments. However, the Velogenic NDV strain AF2240 excelled over the lentogenic NDV V4-UPM with increased oncolytic effects in Saos-2 cells. Conclusion, significance and impact of study: In a nutshell, normoxia or hypoxia microenvironment has little effect on NDV-induced oncolysis of Saos-2 cancer cells which poses as a potential agent for the treatment of resistant cancer.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | hypoxia; Newcastle disease virus; NDV; oncolytic activity; Saos-2 cell; |
| Subjects: | Q Science > QR Microbiology R Medicine > RC Internal medicine |
| Divisions: | Others > Non Sunway Academics Sunway University > School of Medical and Life Sciences [formerly School of Healthcare and Medical Sciences until 2020] > Centre for Virus and Vaccine Research [dissolved] |
| Depositing User: | Ms Yong Yee Chan |
| Date Deposited: | 30 Jul 2024 00:45 |
| Last Modified: | 30 Jul 2024 00:45 |
| URI: | http://eprints.sunway.edu.my/id/eprint/2926 |
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