Bacterial community structure, predicted metabolic activities, and formation of volatile compounds attributed to Malaysian fish sauce flavour

Shanti, Dwita Lestari and Anis Shobirin, Meor Hussin and Shuhaimi, Mustafa and Sew, Yun Shin and Gan, Han Ming * and Amalia, Mohd Hashim and Norhayati, Hussain (2023) Bacterial community structure, predicted metabolic activities, and formation of volatile compounds attributed to Malaysian fish sauce flavour. Food Chemistry, 426. ISSN 0308-8146

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Official URL: https://doi.org/10.1016/j.foodchem.2023.136568

Abstract

The fermentation of Malaysian fish sauce (budu) varies from one to twelve months depending on the producer, resulting in inconsistent quality. The microbiota, their predicted metabolic pathways and volatile metabolites profiles were determined at different stages of budu fermentation. Budu fermented for 1 and 3 months were characterized by the presence of Gram negative Enterobacterales, Gammaproteobacteria, and Fusobacteriaceae, which continuously decrease in abundance over fermentation time. The metabolic pathways prediction grouped 1- and 3- month budu in a cluster enriched with degradation reactions. 6-month budu were dominated by Halanaerobium and Staphylococcus, while the 12-month were dominated by Lentibacillus, Bacilli, and Halomonas. Biosynthesis-type predicted pathways involving protein and lipid derivatives were enriched in 6- and 12-month fermented budu, accumulating 2,6-dimethylpyrazine, methyl 2-ethyldecanoate, 2-phenylacetaldehyde, 3-methylbutanal, and 3-methylbutanoic acid. These compounds may indicate budu maturity and quality. This result may assist as a reference for quality control and fermentation monitoring.

Item Type: Article
Uncontrolled Keywords: 16S metabarcoding; fermented fish; flavour; next generation sequencing; pathway prediction;
Subjects: Q Science > QR Microbiology
S Agriculture > SH Aquaculture. Fisheries. Angling
Divisions: Others > Non Sunway Academics
Sunway University > School of Medical and Life Sciences [formerly School of Healthcare and Medical Sciences until 2020] > Dept. Biological Sciences
Depositing User: Ms Yong Yee Chan
Related URLs:
Date Deposited: 27 Jun 2024 00:32
Last Modified: 27 Jun 2024 00:32
URI: http://eprints.sunway.edu.my/id/eprint/2694

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