Pang, Siew Wai * and Subasri, Armon and Chook, Jack Bee * and Chew, Jactty * and Peh, Kaik-Boo and Lim, Wendy Wan Dee and Peh, Suat Cheng * and Teow, Sin Yeang (2024) Association of Fusobacterium nucleatum infection with the clinicopathological characteristics in colorectal cancer patients. Molecular Biology Reports, 51 (1). ISSN 0301-4851
Full text not available from this repository. (Request a copy)Abstract
Background: Colorectal cancer (CRC) is a global health problem. The gut microbiome is now recognized as an important underlying factor to the initiation and progression of CRC. Fusobacterium nucleatum (FN) is one of the most studied bacteria in the aetiology of CRC. This study provided cohort evidence on the association of FN infection with clinicopathologic features in CRC patients. Methods: We analysed the cancerous and adjacent non-cancerous formalin-fixed paraffin embedded (FFPE) tissue of 83 CRC patients from a single medical centre in Malaysia. TaqMan probe-based qPCR targeting the 16S rRNA gene was used to detect the presence of FN in the extracted FFPE DNA. The differences in FN expression between cancer and non-cancer tissues were evaluated. Association studies between FN infection in the tumour and relative FN abundance with available clinical data were conducted. Results: FN was more abundant in the cancerous tissue compared to non-cancerous tissue (p = 0.0025). FN infection in the tumour was significantly associated with lymph node metastasis (p = 0.047) and cancer staging (p = 0.032), but not with other clinicopathologic variables. In double-positive patients where FN was detected in both cancerous and non-cancerous tissue, the expression fold-change of FN, calculated using 2-ΔΔCT formula, was significantly higher in patients with tumour size equal to or greater than 5 cm (p = 0.033) and in KRAS-mutated patients (p = 0.046). Conclusions: FN is enriched in CRC tumour tissue and is associated with tumour size, lymph node metastasis, cancer staging, and KRAS mutation in this single-centre small cohort study.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | cancerous; clinicopathological features; colorectal cancer; fusobacterium nucleatum; Malaysia; non-cancerous |
| Subjects: | R Medicine > RC Internal medicine |
| Divisions: | Others > Non Sunway Academics Sunway University > School of Medical and Life Sciences [formerly School of Healthcare and Medical Sciences until 2020] > Department of Medical Sciences Sunway University > School of Medical and Life Sciences [formerly School of Healthcare and Medical Sciences until 2020] > Dept. Biological Sciences |
| Depositing User: | Ms Yong Yee Chan |
| Related URLs: | |
| Date Deposited: | 12 Jun 2024 01:24 |
| Last Modified: | 12 Jun 2024 01:24 |
| URI: | http://eprints.sunway.edu.my/id/eprint/2665 |
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