Choo, Suet Yee (2023) Neuroprotective effect of metallothionein against rotenone- and copper-induced toxicity in human dopaminergic SH-SY5Y cells. Masters thesis, Sunway University.
Full text not available from this repository.Abstract
Parkinson’s disease (PD) is characterized by depletion of dopaminergic neurons in the substantia nigra and reduction of dopamine levels in the striatum. Exposure to agricultural pesticides, toxins and metals has been found to induce oxidative stress and toxicity in the brain, associated with the PD pathogenesis. Metallothionein (MT) is a family of metal ion-binding proteins which scavenges free radicals and toxic metal in the nervous system. Although studies have reported the protective role of MTs in neurodegenerative diseases, the underlying mechanism of neuroprotective effects of MT isoform 2 (MT-2) has not been fully studied in PD-associated pathogenesis. This project aims to elucidate the protective effect of recombinant human Metallothionein-2 (hMT-2) protein against rotenone- and metal-induced toxicity in human SH-SY5Y cells as an in vitro PD model. Cells were then exposed to rotenone and copper (II) sulfate (CuSO4) to induce toxicity and cell death. Cell viability assay, oxidative stress analysis, Hoechst staining, apoptosis detection assays and western blot were further conducted to assess the neuroprotective potential of h-MT2 treatment against rotenone- and CuSO4-induced effect in SH-SY5Y cells. Differentiated SH-SY5Y cells exposed to rotenone and CuSO4 exhibited decreased viability, increased ROS production and increased apoptotic events including increased in caspase activation and Bax/Bcl-2 ratio, establishing the in vitro model associated with PD pathogenesis. Further pre-treatment of hMT-2 was shown to improve cell survival and ameliorate the rotenone- and CuSO4-induced oxidative stress and toxicity in SH-SY5Y cells. These results suggest that exogenous hMT-2 treatment may provide neuroprotection against cellular toxicity related to the pathogenesis of PD in SH-SY5H cells.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | Parkinson’s disease; Metallothionein-2; SH-SY5Y cells; neuroprotection. |
| Subjects: | R Medicine > RC Internal medicine R Medicine > RC Internal medicine > RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry |
| Divisions: | Sunway University > School of Medical and Life Sciences [formerly School of Healthcare and Medical Sciences until 2020] > Dept. Biological Sciences |
| Depositing User: | Ms Yong Yee Chan |
| Date Deposited: | 09 Oct 2023 00:03 |
| Last Modified: | 09 Oct 2023 00:03 |
| URI: | http://eprints.sunway.edu.my/id/eprint/2450 |
Actions (login required)
 |
View Item |
