Abd Khalid, Faisal Zarif (2021) Characterization of Antiviral Peptide SP40 and its analogues against Enterovirus A71. Masters thesis, Sunway University.
Full text not available from this repository.Abstract
Enterovirus A71 (EV-A71) is one of the main causative agents of hand, foot and mouth disease (HFMD). Currently, no clinically approved antiviral is available to treat EV-A71 infection. Previously, SP40 peptide was identified to inhibit EV-A71. However the stability and antiviral activity of the peptide in human serum are yet to be evaluated. In addition, exploration of possible strategies to increase peptide stability such as the substitution of L-amino acids to D-amino acids is warranted. Hence, this study examined the antiviral activity of full D-amino acid substituted SP40 peptide (D-SP40) and two partial D-amino acid substituted SP40 peptides (Partial_D-NH2 and Partial_D-Ac) in comparison to the SP40 peptide comprising L-amino acids (L-SP40). RP-HPLC and LCMS/MS were performed to quantitate the amount of full-length L-SP40 peptide remaining after incubation with human serum and to identify the fragments of L-SP40 peptide after cleavage by the serum proteases, respectively.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | Antiviral Peptide SP40; Enterovirus A71; antiviral agents; Hand, Foot and Mouth Disease (HFMD) |
| Subjects: | Q Science > QR Microbiology > QR355 Virology R Medicine > RS Pharmacy and materia medica |
| Divisions: | Sunway University > School of Engineering and Technology [formerly School of Science and Technology until 2020] > Dept. Biological Sciences moved to SMLS wef 2021 |
| Depositing User: | Ms Yong Yee Chan |
| Date Deposited: | 03 Oct 2023 00:48 |
| Last Modified: | 04 Oct 2023 03:09 |
| URI: | http://eprints.sunway.edu.my/id/eprint/2419 |
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