Antibacterial and cytotoxic effect of honey mediated copper nanoparticles synthesized using ultrasonic assistance

Nur Afini, I. and Shameli, Kamyar and Wong, Magdelyn Mei Theing * and Teow, Sin Yeang * and Chew, Jactty * and Siti Nur Amalina, M. S. (2019) Antibacterial and cytotoxic effect of honey mediated copper nanoparticles synthesized using ultrasonic assistance. Materials Science and Engineering: C, 104. p. 109899. ISSN 09284931

Text Teow Sin Yeang-Antibacterialandcytotoxiceffectofhone-1.pdf - Published Version Restricted to Repository staff only until 18 June 2023. Available under License Creative Commons Attribution Non-commercial. Download (335kB) | Request a copy

Abstract

In this study, a comparative study of effect using honey on copper nanoparticles (Cu-NPs) via simple, environmentally friendly process and inexpensive route was reported. Honey and ascorbic acid act as stabilizing and reducing agents with the assistance of sonochemical method. The products were characterized using UV–visible (UV–vis) spectroscopy, X-Ray Diffraction (XRD), High-Resolution Transmission Electron Microscopy (HRTEM), Field-Emission Scanning Electron Microscopy (FESEM) and Fourier Transform Infrared (FTIR) spectroscopy. The reddish brown colour demonstrated the formation of Cu-NPs and UV–visible proved the plasmon resonance of Cu-NPs. XRD also confirmed a highly pure Cu-NPs obtained with absence of copper oxide in which the structure is crystalline. The spherical size of the Cu-NPs was acquire in the presence of honey which is 3.68 ± 0.78 nm with narrow particle distribution. The antibacterial activity was seen against gram-positive and gram-negative bacteria which are Enterococcus faecalis (E. faecalis) and Escherichia coli (E. coli). At higher concentration of Cu-NPs, they were more effective in killing both bacteria. The Cu-NPs without and with honey exhibited toxicities toward normal and cancerous cells. However, Cu-NPs without honey showed more potent killing activity against normal and cancer cells.

Actions (login required)

View Item